Abstract :The highly ordered template of alumina was prepared by means of twice anodic oxidation. The affecting factors involved in the ordered extent and pore size in porous anodic alumina template were concluded from heat process, electrolyte concentration and temperature, etc. Based on the observation and characterization of microstructure and morphology by scanning electron microscopy and transmission electron microscopy , the mechanisms were discussed about order pore of nucleation and growth and the ordered property leaded by self-organized growth. The development of preparation and applications of anodic alumina template was reviewed in recent years.